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NUMA obliviousness through memory mapping. With the rise of multi-socket multi-core CPUs a lot of effort is being put into how to best exploit their abundant CPU power.

In a shared memory setting the multi-socket CPUs are equipped with their own memory module, and access memory modules across sockets in a non-uniform access pattern NUMA.

The thesis analyses various design decisions of the implementation of NUMA support -- introducing the hardware topology into the kernel data structures, propagating this information to user space, thread affinity to Identification and characterization of novel NuMA isoforms. The large nuclear mitotic apparatus NuMA has been investigated for over 30 years with functions related to the formation and maintenance of mitotic spindle poles during mitosis. However, the existence and functions of NuMA isoforms generated by alternative splicing remains unclear.

In the present work, we show that at least seven NuMA isoforms categorized into long, middle and short groups generated by alternative splicing from a common NuMA mRNA precursor were discovered in HeLa cells and these isoforms differ mainly at the carboxyl terminus and the coiled-coil domains. Furthermore, full-length coding sequences of long and middle NuMA obtained by using fusion PCR were constructed into GFP-tagged vector to illustrate their cellular localization.

Long NuMA mainly localized in the nucleus with absence from nucleoli during interphase and translocated to the spindle poles in mitosis.

However, expression of NuMA short isoforms revealed a distinct subcellular localization. Short NuMA were present in the cytosol during the whole cycle, without colocalization with mitotic apparatus.

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